Genomic DNA sequencing

First, as for any of our sequencing services, our wet-lab team performs a quality analysis of the DNA samples provided to us by you. Only when the provided genomic DNA is of sufficient quality and quantity will we proceed with the next steps. In case the DNA provided to us is of insufficient quality and/or quantity, we will contact you and discuss how to proceed.

Next, for the Oxford Nanopore Technologies based WGS, we prepare PCR-free sequencing libraries from the DNA by using a ligation-based approach, offering a great control over library fragment size. After another quality assessment step these libraries are sequenced on our Oxford Nanopore sequencers. The technical quality of the sequencing run is monitored in real time.

When starting genomic DNA is limited (1ng – 100ng), a PCR-based library preparation is also available.

For Illumina based whole genome sequencing, we perform a random fragmentation of the genomic DNA samples followed by size selection of the resulting fragments. This is followed by a second quality control step in which the resulting DNA concentration is determined and the DNA is checked for degradation.
In the following steps, sequencing libraries are prepared from the fragmented DNA by adding indexes and primer adapters. After another quality assessment step these libraries are sequenced on our Illumina sequencers. The technical quality of the sequencing run is monitored in real time.

Raw sequencing data can be transferred to you via the ohmX.bio server (FTP download) or the Illumina BaseSpace platform.