OHMX.bio provides high quality services equipped with start-of-the-art technologies. We offer start-to-finish solutions for all of your -omics needs.
Translatomics or ribosome profiling (RIBO-seq) is the genome-wide measurement of the mRNA protected by translating ribosomes. We can provide you with a genome-wide snapshot of mRNA that is being translated.
Transcriptomics is the study of the complete set of RNA transcripts that are produced by cells under specific conditions. Total RNA, messenger RNA (mRNA), non-coding RNA can be sequenced and quantified.
Genomics is the study of the full genomic material of a cell, tissue or organism. Full genomes or targeted regions thereof can be measured using state-of-the-art next and third generation sequencing instruments.
OhmX.bio possesses state-of-the-art sample preparation equipment that allows a lot of different sample preparations in an automated, efficient and accurate way.
The Diagenode IP-Star is a compact automated system that provides the flexibility needed to change multiple parameters in the protocols to achieve optimal experimental conditions. It allows for high-throughput sample preparation compatible with popular downstream applications such as qPCR and next generation sequencing platforms. In addition to automated ChIP and DNA methylation protocols, this system contains a wide variety of protocols including next generation sequencing library preparation and sample purifications.
The Covaris S2 focused-ultrasonicator is a versatile, high power system engineered for pre-analytical sample processing for numerous biological and chemical applications including, DNA, RNA, and chromatin shearing, tissue homogenization, cell lysis, compound dissolution, and particle micronization. The computer controlled device allows for shearing at any set fragment size.
OhmX.bio is fully equipped with the latest technologies to process a high throughput of samples with the most diverse library preparation protocols. Virtually all steps are quality controlled using Qubit Fluorometric Quantitation (Thermo Fisher Scientific), q-PCR, Bioanalyser (Agilent Genomics), LabChip (PerkinElmer), etc.
We offer a wide range of sequencing apparatus allowing to sequence at the scale and scope of every project.
The Oxford Nanopore sequencers make us of a membrane containing nano-scale holes or nanopores. By setting a voltage across this membrane, strands of DNA or RNA pass through the nanopores making it possible to detect an ionic current. Changes in these currents can be used to identify that molecule.
This portable real-time device for DNA and RNA sequencing allows us to generate 10 – 30 Gb of data. The Oxford Nanopore sequencing technology will be applied for our genomics and epigenomics work, seen its clear advantages as for instance the sequencing of (ultra-) long reads up to 2 Megabases, the ability to perform direct DNA sequencing and the simple and rapid library preparation. Different types of double stranded DNA can be measured as for example genomic DNA (gDNA), complement DNA (cDNA) or amplicons.
The GridION X5 is a compact benchtop system designed to run and analyse up to five MinION Flow Cells. The sequencer has a very flexible setup that allows up to five experiments to be run concurrently or individually. The current chemistry and software release enables generation of up to 150 Gb of data during one run. The data is analysed in real time.
The MiSeq benchtop sequencer is well suited for smaller projects and thus allows for more focused applications such as targeted gene sequencing, metagenomics, small genome and transcriptome sequencing, and targeted gene expression. Generating 1 million up to 25 million reads, the MiSeq sequencer is ideal for reduced representation studies and narrowly focused applications.
With an output of up to 400 million reads, our Illumina NextSeq 500 sequencer is more suited for larger study designs. It is the only benchtop sequencer that can sequence a 30x coverage human genome in one run. The NextSeq 500 is easily configured providing researchers perfect scalability for mid-sized to large projects at maximal operational efficiency.
The HiSeq 3000 System is a powerful and efficient ultra-high-throughput sequencing system that, with an output of up to 750 gigabase (Gb) of data, is ideally suited for large-scale genomics projects.