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OHMX_High-throughput OMICS solutions

Directional
RNA sequencing

Directional RNA sequencing

We offer directional RNA sequencing of total RNA, mRNA, and miRNA (small RNA). RNA sequencing not only provides comprehensive knowledge about all transcripts present in the sample, but also quantifies the abundance of each transcript. With directional RNA sequencing, information is retained concerning the strand (sense vs anti-sense) the RNA originated from. The choice for mRNA, miRNA or total RNA sequencing depends on your setting and question you want to answer.

Directional RNA-seq advantages

Full transcriptome from every species

Differential gene/transcript expression analysis

Directionality of the RNA is retained (sense vs antisense strand)

Directional RNA-seq workflow

You provide us with extracted total RNA samples. After an initial quality check of the RNA where we determine the concentration and integrity of the RNA, sequencable libraries from the samples are constructed. We only continue with samples that passed this initial quality check. If for some reason certain samples did not pass this initial check, we will contact you to discuss on how to proceed.

Depending on the kind of RNA-seq a size-selection step is performed. Also, the library preparation may vary depending on the kind of RNA-seq. The prepared libraries undergo a final quality check before being loaded for sequencing on one of our Illumina sequencers. The technical quality of the sequencing run is monitored in real time.

Raw sequencing data can be transferred to you via the ohmX.bio server (FTP download) or the Illumina BaseSpace platform.

What do our customers say

  • "We were very well assisted and helped by the expert team of OHMX in our sequencing projects. Definitely when things get challenging (advanced sequencing protocols, low input quantities), the team thrives."

    Cedric Bogaert

  • "We are very pleased with the way how the company is willing to do customized projects. The team takes responsibility for their work and results they provide. The communication with the team is very easy and fast. We wish the company to grow and flourish. Thank you for the excellent work!"

    Anya Goropashnaya

  • “OHMX.bio is understanding very well the need for data quality control and the validation of the different steps in the transcriptomics analysis workflow. That is of great importance to us and created a lot of trust in the services they offer. They are flexible in their project approach and design and provide additional ad hoc requirements for sequencing and data delivery.”

    Sahar Hosseinian Ehrensberger (CTO and General Manager)

  • “OHMX.bio has a broad expertise in multi-omics. Their laboratories are equipped with state-of-the-art analysis platforms and the company also has a strong bioinformatics team. So, outsourcing to one party with expertise in both RNA-seq, RIBO-seq & proteomics under one roof, made fully sense to us. Because of their strong know-how working with big data, they made a compilation & analysis of all our data over a large time scale, and this was impressive.”

    Glenn Pauwelyn (Head of Research and Development)

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  • Belgium wet lab
Campus proeftuin – Building N1 Proeftuinstraat 86, 9000 Ghent