Applications

Translational analysis

Ribosome profiling (also called RIBO-seq), uses specialized messenger RNA (mRNA) sequencing to determine which mRNAs are being actively translated. It produces a “global snapshot” of all the mRNA that is being translated by all active ribosomes in a sample at a particular moment, known as a translatome.

 

Advantages

  • Ribosome profiling is highly beneficial as NGS alternative to or complementary to MS-based protein and peptide identification and will develop into a common practice for next-generation proteomics
  • With ribosome profiling the translational control is investigated and the gene expression is measured at the translational level
  • It allows to determine the rate of protein synthesis over a large dynamic range

 

Applications

 

Ribosome profiling can be applied to animals, other eukaryotes, plants, and bacteria.

 

  • Identification of translated sequences within the complex transcriptome
  • Mapping sites of translation initiation (TIS)
  • Measurement of differential gene expression at the level of mRNA translation
  • Identification of novel protein coding genes and ribosome pausing
Related services
Translatomics_ohmx
Bioinformatics_ohmx
Transcriptomics_ohmx
Selected publications
PROTEOFORMER 2.0: further developments in the ribosome profiling-assisted proteogenomic hunt for new proteoforms.

Steven Verbruggen, Elvis Ndah, Wim Van Criekinge, Siegfried Gessulat, Bernhard Kuster, Mathias Wilhelm, Petra Van Damme and Gerben Menschaert. (2019).

Molecular & Cellular Proteomics18(8 suppl 1), S126-S140

https://doi.org/10.1074/mcp.RA118.001218

N-terminal proteomics and ribosome profiling provide a comprehensive view of the alternative translation initiation landscape in mice and men.

Van Damme, P., Gawron, D., Van Criekinge, W., & Menschaert, G. (2014).

Molecular & Cellular Proteomics13(5), 1245-1261.

https://doi.org/10.1074/mcp.M113.036442