Applications
Ribosome profiling (also called RIBO-seq), uses specialized messenger RNA (mRNA) sequencing to determine which mRNAs are being actively translated. It produces a “global snapshot” of all the mRNA that is being translated by all active ribosomes in a sample at a particular moment, known as a translatome.
Advantages
- Ribosome profiling is highly beneficial as NGS alternative to or complementary to MS-based protein and peptide identification and will develop into a common practice for next-generation proteomics
- With ribosome profiling the translational control is investigated and the gene expression is measured at the translational level
- It allows to determine the rate of protein synthesis over a large dynamic range
Applications
Ribosome profiling can be applied to animals, other eukaryotes, plants, and bacteria.
- Identification of translated sequences within the complex transcriptome
- Mapping sites of translation initiation (TIS)
- Measurement of differential gene expression at the level of mRNA translation
- Identification of novel protein coding genes and ribosome pausing
Related services
Selected publications
PROTEOFORMER 2.0: further developments in the ribosome profiling-assisted proteogenomic hunt for new proteoforms.
Steven Verbruggen, Elvis Ndah, Wim Van Criekinge, Siegfried Gessulat, Bernhard Kuster, Mathias Wilhelm, Petra Van Damme and Gerben Menschaert. (2019).
Molecular & Cellular Proteomics, 18(8 suppl 1), S126-S140
N-terminal proteomics and ribosome profiling provide a comprehensive view of the alternative translation initiation landscape in mice and men.
Van Damme, P., Gawron, D., Van Criekinge, W., & Menschaert, G. (2014).
Molecular & Cellular Proteomics, 13(5), 1245-1261.