Ribosome profiling (also called RIBO-seq), uses specialized messenger RNA (mRNA) sequencing to determine which mRNAs are being actively translated. It produces a “global snapshot” of all the mRNA that is being translated by all active ribosomes in a sample at a particular moment, known as a translatome.
Ribosome profiling can be applied to animals, other eukaryotes, plants, and bacteria.
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