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Translatomics

Translatomics

Translatomics
“Mapping the translational landscape”

With our in-house developed high performant ribosome profiling service, we provide a genome-wide assessment of the mRNA targeted by translating ribosomes. This highly sensitive sequencing-based technology unravels the translatome, bridging the fields of transcriptomics and proteomics.

We offer competitive pricing, fast turn-around, highest quality of execution, and customized project design and execution to fit your needs from data collection, through data analysis, to result summary.

Ribo-seq technology

Discover our fast and reliable ribosome profiling service.

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Ribo-seq bioinformatics

We have developed in house bioinformatics pipelines for QC, preprocessing/cleaning, mapping and differential analysis.

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Applications
Applications

Ribosome footprint profiling can be applied to measure mechanisms in protein synthesis and its regulation in the cell as well as related co-translational processes:

 

  • Quantification of gene-specific translation: the number of ribosomes translating a reading frame determines the number of footprints generated in a profiling experiment, thus also the amount of encoded protein that is synthesized.
  • Discovery of non-canonical translation events: RIBO-seq enables to unravel multiple ORFs within a gene locus. Often, ribosome profiling footprints are mapped to 5’ UTR regions hinting towards translation of an upstream ORF (uORF), next to the downstream canonical ORF. Likewise, ORF-delineation is made possible in non-coding RNA genes, pointing to (small) ORFs in so-called non-coding genes. Protein variants, as for example protein extensions and truncations or different isoforms are also detectable with RIBO-seq. These alternative ORFs often have a near-cognate start site.
  • Ribosome elongation speed and pausing can also be investigated with this technique. Several factors as tRNA availability or modification and/or amino acid limitation influence the speed of translation. Slow elongation will manifest itself in accumulated higher ribosome occupancy as compared to regions where faster elongations is occurring.
  • Translation regulation by microRNAs and RNA-binding proteins can also be looked into with this technique.
  • Mechanisms of the translation initiation, and the initiation factors that play are role herein, but also the translation termination, recycling and quality control can be investigated.

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