Quantitative PCR

Quantitative PCR

Quantitative Polymerase Chain Reaction (qPCR), also known as Real-Time PCR, is a highly sensitive and precise molecular method for quantifying specific nucleic acid targets. At OHMX.bio, we leverage advanced qPCR platforms and expert assay design to provide robust and reliable solutions for a wide range of research and clinical applications. Our services deliver accurate, reproducible, and rapid results, making qPCR an essential tool for gene expression analysis, genetic variation detection, and biomarker validation.

qPCR capabilities

qPCR in clinical trials

Custom assay development

Comprehensive qPCR capabilities

OHMX.bio offers comprehensive qPCR services designed to meet diverse research needs, from fundamental biological studies to targeted molecular detection. Our capabilities include:

Quantitative Measurement

Precise quantification of DNA or RNA targets across a broad dynamic range, enabling accurate assessment of gene expression levels, viral loads, or copy number variations.

High Sensitivity

Detection of even low-abundance nucleic acid molecules, crucial for early disease detection, minimal residual disease (MRD) monitoring, and analysis of challenging samples.

Versatile Assay Formats

Support for both SYBR Green and probe-based assays, allowing for flexibility in experimental design and multiplexing capabilities for simultaneous detection of multiple targets.

Throughput Options

Our platforms support both 96-well and 384-well formats, accommodating projects of varying scales, from small research batches to high-throughput screening.

OHMX.bio - Innovative omics solutions
OHMX.bio_Innovative omics solutions

qPCR in clinical trials: Scalability and GCLP compliance

For clinical research and diagnostic applications, qPCR offers significant advantages due to its scalability, speed, and quantitative nature. OHMX.bio is a trusted partner for deploying qPCR in clinical trials, ensuring data integrity and regulatory compliance:

Scalable Solutions

Our automated setups and optimized workflows enable efficient, high-throughput sample preparation and analysis, supporting large-cohort clinical studies and rapid turnaround times essential for timely decision-making.

GCLP Compliance

We adhere to Good Clinical Laboratory Practice (GCLP) standards for relevant pipelines, ensuring that our laboratory practices meet the rigorous requirements for clinical-grade testing. This commitment to quality provides the reliability and traceability necessary for regulatory submissions and robust clinical validation.

Biomarker Validation

qPCR is an ideal platform for validating biomarkers identified through discovery-phase omics studies, offering a targeted and cost-effective method for quantifying specific genetic or expression markers in a clinical context.

Custom assay development and optimization

Beyond standard applications, OHMX.bio specializes in developing and optimizing custom qPCR assays tailored to your unique research requirements. Our team of experts provides end-to-end support for custom assay development:

Target-Specific Design

We design and validate custom primers and probes for novel or unique nucleic acid targets, ensuring high specificity and sensitivity for your particular gene or sequence of interest.

Panel Customization

For projects requiring the simultaneous detection of multiple targets, we can design and implement custom pre-spotted plates, enhancing throughput and efficiency for multiplexed detection panels.

Method Optimization

Our scientists work to optimize reaction conditions, ensuring peak performance and reproducibility for your custom assays, adhering to MIQE (Minimum Information for Publication of Quantitative Real-Time PCR Experiments) guidelines for high-quality results.

Partner with OHMX.bio for your Real-Time PCR needs, and leverage our expertise to achieve precise, reliable, and actionable nucleic acid quantification for your research and clinical objectives.
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Let’s get in touch!

Are you curious about how we can work on your -omics challenges together? Fill out the form below!
Our experts will reply within 2 working days to freely discuss your omics project.

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