Ribosomal Profiling

Ribosomal profiling (RIBO-seq)

OHMX.bio delivers cutting-edge ribosome profiling (RIBO-seq) solutions with a proprietary, high-performance pipeline, offering unparalleled insights into translation dynamics. Founded on this groundbreaking technology, OHMX.bio is a globally recognized leader in ribosome profiling, with numerous peer-reviewed publications demonstrating its expertise. With an industry-leading rRNA depletion strategy, OHMX.bio provides the most precise and reliable translatomics data available.
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Understanding ribosome profiling

Ribosome profiling is a next-generation sequencing technique that captures ribosome-protected fragments (RPFs), providing a real-time view of translation across the entire transcriptome. By pinpointing ribosome positions on mRNAs, RIBO-seq enables detailed analysis of translational regulation, ribosome occupancy, and open reading frame (ORF) delineation. This technology bridges the gap between transcriptomics and proteomics, revealing crucial insights into gene expression that RNA sequencing alone cannot provide.
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Tailored ribosome profiling solutions

OHMX.bio offers two distinct ribosome profiling solutions, each designed for specific research objectives:

1. Differential Translation Analysis

This solution focuses on comparing ribosome occupancy across different conditions, enabling researchers to identify genes with altered translation rates.  
  • Designed for differential expression studies at the translational level.
  • Helps uncover translational control mechanisms beyond mRNA abundance.
  • Includes a dedicated advanced bioinformatics package for quantitative RPF analysis, normalization, and statistical comparisons.
  • Optional Matching RNA Sequencing – RNA-seq from the same sample can be included to determine translational efficiency by comparing mRNA abundance with ribosome occupancy.

2. ORF Delineation & Discovery

This approach provides in-depth mapping of ribosome footprints to precisely define known and novel ORFs, including small upstream ORFs (uORFs) and non-canonical translation events.
  • Essential for discovering novel protein-coding regions.
  • Resolves complex translational landscapes with single-nucleotide precision.
  • Includes a specialized advanced bioinformatics package for frame analysis, codon periodicity detection, and novel ORF
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Unparalleled bioinformatics expertise

OHMX.bio provides two advanced bioinformatics packages, each tailored to support one of the ribosome profiling pipelines:

Differential Translation Analysis Package

Focuses on quantitative RPF analysis, normalization, and statistical comparisons across conditions.

ORF Delineation & Discovery Package

Designed for high-resolution mapping of ribosome footprints, identifying novel ORFs, and frame analysis.

With a proprietary bioinformatics pipeline and industry-leading rRNA depletion technology, OHMX.bio ensures the highest signal-to-noise ratio, delivering the most precise and interpretable data.
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Sample compatibility

OHMX.bio has successfully optimized its ribosome profiling protocols across a wide range of sample types, including:

Cell lines

Adherent and suspension cultures.

Tissue samples

Various tissue types, both frozen and fresh.

Liquid biopsies

Adapted protocols for challenging sample types.

Plants

Specialized procedures for plant-derived samples.

With extensive experience performing ribosome profiling on diverse biological systems, OHMX.bio provides detailed guidelines for sample preparation, including ribosomal stalling protocols to preserve active translation states. Specific recommendations for cycloheximide treatment and cell lysis are available to guarantee high-quality RPF isolation.
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Why choose OHMX.bio?

With a foundation in ribosome profiling, OHMX.bio is the trusted expert in translatomics. Leveraging proprietary methodologies, deep bioinformatics expertise, and a commitment to scientific excellence, OHMX.bio delivers the most accurate and insightful RIBO-seq data for cutting-edge research.

Contact OHMX.bio today to discuss your ribosome profiling needs and take your translational research to the next level.

Let’s get in touch!

Are you curious about how we can work on your -omics challenges together? Fill out the form below!
Our experts will reply within 2 working days to freely discuss your omics project.

Our publications using our ribo-seq services

  1. Clauwaert, Jim, et al. “Deep learning to decode sites of RNA translation in normal and cancerous tissues.” Nature Communications 16.1 (2025): 1275.

  2. Deutsch, Eric W., et al. “High-quality peptide evidence for annotating non-canonical open reading frames as human proteins.” BioRxiv (2024).

  3. De Paolis, Elisa, et al. “Characterization of shared neoantigens landscape in Mismatch Repair Deficient Endometrial Cancer.” NPJ Precision Oncology 8.1 (2024): 283.

  4. Urbani, Andrea, et al. “Characterization of shared neoantigens in Endometrial Cancer with Microsatellite Instability.” (2024).