Process up to 96 samples or one million cells in one experiment.

Fix cells or nuclei to lock in the biology until your experiment is ready. In timecourse studies, avoid uncertainty and remove batch effects by running samples collected on different days together. Samples can be stored up to 6 months.

Cells are distributed into wells and receive a sample specific barcode through an in cell reverse transcription reaction. Cells from each well are then pooled together. This is repeated two more times, after which the pooled cells are divided into sublibraries  where lysis is performed and a fourth sublibrary specific barcode is attached.

In the library preparation, adapters are attached to prepare for sequencing on next- or third-gen sequening technologies (e.g. Illumina, Oxford Nanopore Technologies, Element Biosciences)

Data analysis consists of Quality Control, generating a gene-cell count matrix and downstream analysis using tools such as Seurat or Scanpy