Biological samples, such as cell lysates or tissue extracts, are prepared and enriched for phosphopeptides using specific affinity-based techniques to enhance detection sensitivity.
Enriched phosphopeptides are analyzed using high-resolution mass spectrometry, enabling precise identification and quantification of phosphorylation sites across the proteome.
Advanced bioinformatics tools are employed to process the mass spectrometry data, providing comprehensive phosphorylation site mapping and quantification.
