at single-nucleotide resolution.
to analyze transcript modifications.
to resolve complex regions and repetitive elements.
Enables direct detection of multiple base modifications, such as 5mC and 6mA, without chemical conversion. ONT is particularly valuable for detecting modifications in both DNA and RNA, making it a versatile tool for epigenetic studies. Its long-read capabilities allow for better resolution of complex genomic regions, repetitive sequences, and phased methylation patterns. However, ONT has a slightly higher error rate compared to short-read sequencing methods, which can be mitigated with optimized data processing and coverage.
Offers high accuracy in detecting 5mC modifications but requires chemical treatment, which converts unmethylated cytosines to uracil while leaving methylated cytosines unchanged. This process can introduce DNA degradation and sequencing biases, potentially affecting data quality. Bisulfite sequencing provides high-resolution methylation mapping at a lower per-base error rate than ONT but does not detect RNA modifications or other epigenetic marks.
Identify methylation changes linked to tumor progression.
Study the role of epigenetic modifications in neurodegeneration and aging-related diseases.
Explore how epigenetic mechanisms shape gene expression during development.
Investigate epigenetic markers for personalized medicine.
