Microbiome analysis

Nanopore sequencing yields long reads that can deliver enhanced genome assemblies, accurate identification of closely related species based on full 16S and 23S typing, and unambiguous analysis of full-length RNA transcripts from mixed microbial samples (metatranscriptomis). Also the epitranscriptome (RNA modifications) can be charted from the microbiome population.

Advantages:

• Abundance measure without PCR bias
• Rapid workflow allowing multiplexing
• Targeted long read approaches for informative genes (full 16S and 23S rRNA)
• Metagenome and metatranscriptome analysis
• Direct DNA or RNA sequencing enables base modification identification

Applications:

• Gut microbiome
• Marine microbiome
• Microbiome culture characterization