Translation profiling

Ribosome profiling (also called RIBO-seq), uses specialized messenger RNA (mRNA) sequencing to determine which mRNAs are being actively translated. It produces a “global snapshot” of all the mRNA that is being translated by all active ribosomes in a sample at a particular moment, known as a translatome (translatomics).

Advantages

• Ribosome profiling is highly beneficial as NGS alternative to or complementary to MS-based protein and peptide identification and will develop into a common practice for next-generation proteomics
• With ribosome profiling the translational control is investigated and the gene expression is measured at the translational level
• It allows to determine the rate of protein synthesis over a large dynamic range

Applications

Ribosome profiling can be applied to animals, other eukaryotes, plants, and bacteria.

• Identification of translated sequences within the complex transcriptome
• Mapping sites of translation initiation (TIS)
• Measurement of differential gene expression at the level of mRNA translation
• Identification of novel protein coding genes and ribosome pausing